Automated Peptide Mapping and Intact Mass Analysis Data Processing Workflows for Streamlined MS-Based Characterization of AAV Capsids

Genedata Biopharma Partner Symposium, Basel, Switzerland
March 6, 2024

Adeno-associated viruses (AAVs) are a leading gene delivery platform due to their high infectivity, low immune response, and high overall safety compared to other viral vectors. However, to ensure vector stability and potency of gene therapy products, characterization of AAV capsids is crucial during candidate selection and process development. Mass spectrometry-based analytical methods enable in-depth capsid characterization at the protein and peptide level — for example, accurate assessment and monitoring of deamidation and phosphorylation at potential stability hot spots — and consequently are increasingly being used for serotype confirmation and heterogeneity characterization. We present automated peptide mapping and intact mass analysis workflows for MS data processing, analysis, and reporting that streamline MS-based characterization of AAV capsids and facilitate downstream deployment of novel cell and gene therapies.