ウェビナー動画：A*STAR、エタネルセプトのN-/O-結合型糖鎖部位の包括的なMS特性解析を可能にするデータ解析戦略
複雑な糖鎖プロファイルを持つエタネルセプトにとって、質量分析は特性解析するのに最適な手法ですが、最先端のMS機器から出力されるデータが複雑なため、処理・解析・レポーティングの上で、いくつかの課題が存在します。本動画では、A*STAR Bioprocessing Technology Institute（BTI）のXuezhi Bi氏が登壇し、Genedata Expressionistを活用することで、エタネルセプトの包括的かつ緻密な特性解析を実現した事例を紹介しています。
The biotherapeutic recombinant Fc-fusion protein Etanercept has a more complex and heterogeneous glycosylation profile than conventional antibody-based therapeutics. In addition to a complex N-linked glycan profile, Etanercept also possesses multiple potential sites for O-linked glycosylation.
Mass spectrometry is the method of choice for determining the nature, multiplicity, and heterogeneity of such glycosylation sites, but the complex data produced by cutting-edge MS instrumentation presents several processing, analysis, and reporting challenges.
In this webinar you will learn how Dr. Bi and his team used a range of MS-based analytical methods to achieve a comprehensive, in-depth characterization of Etanercept. Automated MS data processing using a dedicated and optimized workflow for analysis and reporting enabled unambiguous identification of both N- and O-linked glycosylation sites, while utilizing customized glycan libraries and a novel search strategy drastically increased the confidence of the results, enabling deeper insights than previously obtainable.
Who Should Watch
Biopharma analytical scientists and managers who are looking to automate mass spectrometry-based analytical workflows for characterization and quality monitoring of biotherapeutics.
