ポスター：ミドルダウンシーケンシングを活用して抗体医薬品のインタクトプロテイン解析を検証する統合データワークフロー

ASMS 2023, Houston, TX, USA
June 8, 2023

While mass spectrometry offers many advantages in biopharmaceutical characterization, methods based on peptide mapping are labor intensive and time-consuming, generating large data sets that require trained and skilled manual interpretation. In addition, the sample preparation process of peptide mapping can create artifactual modifications and deliver results that are not a true reflection of the native state of the molecule. A faster and less complex alternative to characterizing antibodies by peptide mapping is middle-down intact mass analysis, which utilizes novel enzymes that cleave antibodies at a highly specific site above or below the hinge, resulting in well-defined fragments that are amenable to characterization by LC-MS/MS.

We present a fully automated data processing workflow that enables fast and efficient middle-down protein sequencing and simultaneous intact mass analysis of protein biotherapeutics.