HILIC MS-enabled sMAM: Advancing the Future of Biotherapeutic Characterization

Learn how Dr. Benqian Wei, Senior Scientist from Merck & Co. applies hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS)-based subunit-level multi-attribute method (sMAM) workflows with Genedata for biotherapeutic characterization. 

Using IgG and Fc-fusion protein case studies, the presentation will show how this approach improves chromatographic separation and monitoring of important product quality attributes (PQAs), including O- and N-glycoforms, deamidation, oxidation, and succinimide formation. 

Attendees will learn how subunit-level HILIC-MS can shorten sample preparation and LC-MS analysis compared with peptide-based MAM while preserving quantitative performance for selected product quality attributes, supporting faster and more streamlined characterization of complex biotherapeutics.