Workflow Automation for High-Throughput Spectral Shift Analysis

October 1, 2025

High Throughput Spectral Shift (HT-SpS) is a cutting-edge biophysical screening technology that enables direct detection of binders and allosteric modulators, including both small molecules and biologics. For the first time, hits can be identified through direct biophysical measurements at the earliest stages of drug discovery. The NanoTemper Dianthus uHTS is a high-performance instrument based on SpS technology, capable of measuring a full 1536-plate in approximately 7 minutes.

Here we present an automated end-to-end workflow in Genedata Screener^®, part of the Genedata Biopharma Platform, that enables HT-SpS hit detection with unprecedented throughput. Genedata Screener fully automates the entire analysis workflow for Dianthus uHTS data, including data loading, processing, quality control, result calculation, hit identification, and reporting to downstream applications. It efficiently handles diverse (HT-)SpS datasets, enables interactive review of raw spectral scan graphs at any step, performs automated outlier detection, provides robust QC metrics, and supports sample ranking via direct affinity constant determination.  With the addition of SpS to the broad suite of assay technologies covered, Genedata Screener thus continues to accelerate the identification of new molecules from the beginning of the discovery cycle.