Automated Analysis of High-Throughput Protein Stability For Antibody Developability Assessment

October 21, 2025

Therapeutic large molecules, such as antibodies, are an increasingly important and rapidly growing segment of the pharmaceutical industry. For many of these biologic candidates,  protein stability presents a significant challenge in early screening stages that can limit their advancement to clinical use. Thus, assessing protein stability using multiple parameters is considered beneficial during their development. Dynamic Light Scattering (DLS) and nano Differential Scanning Fluorimetry (nanoDSF) can be performed simultaneously with instruments such as the NanoTemper Prometheus Panta for label-free measurements of thermal stability, aggregation, particle size, and dispersity. These high-throughput techniques generate substantial amounts of data, likely requiring extensive manual review and analysis. Genedata Screener provides an automated solution for these high-throughput workflows streamlining the analysis of protein stability data. Additionally, Screener integrates seamlessly with other assay datasets to deliver comprehensive insights into compound developability.