HILIC MS-Enabled Subunit Level MAM - Advancing the Future of Biotherapeutic Characterization

Learn how Dr. Benqian Wei, Senior Scientist from Merck & Co. applies hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS)-based subunit-level multi-attribute method (sMAM) workflows in Genedata Expressionist for biotherapeutic characterization. Using IgG and Fc-fusion protein case studies, the presentation will show how this approach improves chromatographic separation and monitoring of important product quality attributes (PQAs), including O- and N-glycoforms, deamidation, oxidation, and succinimide formation. 

Attendees will learn how to: 

• Monitormultiple PQAs within a single HILIC-MS-based subunit-level MAM assay
• Shorten sample preparation and LC-MS analysis compared with peptide-based MAM workflows
• Improveseparation and monitoring of post translational modifications (PTMs), O-glycoforms, and N-glycoforms in complex biotherapeutics
• Evaluate relative PQA abundance with reproducible quantitative performance
• Streamline biotherapeutic characterization with Genedata Expressionist-supported sMAM workflows