Biophysical screening utilizes a wide range of technologies, such as impedance, surface plasmon resonance (SPR), thermal shift analysis (TSA), optical waveguide, and many others. In some cases, the screening is on a smaller scale and answers complex biophysical questions; other times the questions are simpler and the scale larger. Genedata Screener® supports all biophysical screening methods that are plate-based and some that are not, providing benefits that vary slightly depending on the challenges of each individual technology.
Standardized and flexible methods
Some challenges encountered in biophysical screening stem from the fact that many of these technologies are only recently applied to screening. For example, each instrument typically has methods suitable for time-series analysis. But as best practices are not yet available, not all instruments within one technology provide the same range of methods and it is often difficult or impossible to add new analysis methods. In addition, many instruments offer only manual step-by-step analysis, creating time-sinks and error-prone workflows. When this is the case, Genedata Screener offers great advantages in the form of standardized and automated methods together with the option to add new methods and modify existing ones. This can lead to time-savings of a factor up to ten (see for example our application note on the implementation of Genedata Screener for a TSA workflow at a major pharma customer, or the JBS article on our collaboration on SPR with AstraZeneca).
Immediate propagation of changes
As they are new to screening, most instruments can interactively analyze only one plate at a time. Multiple-plate experiments must be analyzed in batch mode, without the possibility to review the results from all plates together. This often means that the results of the initial analysis performed on the instrument can only be properly reviewed after export to a downstream analysis software. If something must be changed, the user has to go back to the instrument software, correct the erroneous parameter, save the results, re-export to the downstream software, and re-do the analysis. Genedata Screener imports and analyzes all time-traces in each well, which means there is no need to go back to the instrument software once the initial import is completed. Because aggregation, calculation, and analysis can be done directly in Screener, any adjustments needed are performed directly on the relevant step with immediate effect on the results. Genedata Screener is made for high-volume analysis and analyzes unlimited numbers of plates simultaneously, allowing side-by-side comparisons between individual results, or across plates or even batches. By having complete access to all data side-by-side, suspicious results can often be explained immediately, and traces that are similar can be grouped and tagged for closer review.
One platform for all biophysical screening data analysis
However, one of the main benefits of Genedata Screener for Biophysical Screening is that it offers a single data analysis platform for many different instruments of various types and brands, making it possible to create one single workflow for a screening lab. Screener integrates all technologies, standardizes ways of importing raw data, performs quality control, calculates and evaluates final results, and allows instant submission to central repositories.
Below are some examples of supported technologies and applications. For more information on each technology, take a look at the webinars and documents below or contact us directly.