Utilizing Cole–Cole parameters for in-line feedback: Cell culture process adjustments based on cell health

Biotechnology Progress
December 28, 2025

In the biopharmaceutical industry, effective process control strategies are essential for enhancing drug substance quality and yield. This study presents dielectric spectroscopy as a novel approach for in-line monitoring of cell apoptosis, enabling earlier detection of apoptotic events and related cellular changes. 

Genedata Expressionist^® empowered the analysis and postprocessing of proteomics datasets, enabling real-time monitoring of cell death in CHO cell cultures during batch and perfusion processes. This robust workflow enabled precise pathway enrichment interpretation, supporting a deeper understanding of cellular stress responses, and optimizing bioprocess strategies for improved performance and resource utilization.