An Integrated High-Throughput Workflow for Robust SPR-Based Biotherapeutic Characterization

January 29, 2026

Surface plasmon resonance (SPR) is a sensitive, real-time, label-free technique for biomolecular interaction analysis (BIA), making it a key technology for biopharmaceutical discovery and development. Bruker’s Triceratops SPR #64 introduces an innovative rotatable microfluidic design that allows simultaneous measurement of 64 sensor spots, enabling kinetic characterization at high throughput. In addition to conventional Multi Injection Cycle Kinetics (MICK) the Triceratops SPR #64 supports also Titration Cycle Kinetics (TCK) suitable for high-affinity (non-regenerating) interactions and Single Injection Cycle Kinetics (SICK), which allows the full kinetic characterization of 8 targets in a single injection. All assay formats can be analyzed using streamlined workflows for high-throughput kinetic characterization in Genedata Screener^®. The software offers advantages in automation, data standardization, scalability, and collaboration across an enterprise setting.
Here, we showcase the versatility of the Triceratops and demonstrate robust and reproducible kinetic parameter determination using Genedata Screener®. We thus present a state-of-the art, comprehensive solution for SPR-based characterization of biopharmaceuticals.