Integrated HRPF Workflows for Structural MS Data Processing

May 20, 2026

Hydroxyl radical protein footprinting (HRPF) is a mass spectrometry–based approach for characterizing protein higher‑order structure by measuring changes in solvent accessibility at the residue level, but consistent radical delivery and scalable data interpretation remain key challenges. Variability in hydroxyl radical exposure and the complexity of HRPF datasets can limit reproducibility and complicate structural comparisons across conditions.

This poster presents an integrated HRPF workflow developed in collaboration with GenNext that combines the AutoFox^® Protein Footprinting System with Genedata Expressionist^® to enable automated, end‑to‑end data processing for MS‑based structural biology. You will learn how controlled radical generation with real‑time dosimetry and automated preprocessing, alignment, and statistical analysis improve reproducibility of HRPF experiments, support robust residue‑level comparison of oxidation across conditions, and reduce manual effort in large‑scale structural MS studies. It also demonstrates how integrated, traceable workflows enable scalable structural characterization and provide a strong foundation for comparative structural analysis. Designed for scientists working in structural MS and protein characterization.