ポスター：異なるMS/MSフラグメンテーションデータを組み合わせた包括的なミドルダウン解析のためのデータ処理ワークフロー

ASMS 2024, Anaheim, CA, USA
June 2, 2024

Middle-down protein sequencing of intact or partially digested therapeutic antibodies offers a faster and less complex alternative to characterizing antibodies by peptide mapping and provides more information than intact mass analysis. By utilizing novel enzymes that cleave antibodies to generate well-defined fragments that are amenable to characterization by LC-MS/MS, middle-down protein sequencing facilitates validation of modifications detected at the peptide level. A key requirement of these analyses is high-confidence identification of the ions used for sequencing. Combining two orthogonal dissociation methods (collision-induced [CID] and electron-activated [EAD] dissociation) provides complementary fragmentation patterns that increase overall sequence coverage and increased confidence in each fragment ion identification.