Poster: Integrated HCS image and data analysis workflow, applied to a Transfluor G protein-coupled receptor screen; Joint poster with Cellomics, SBS 2007
SBS 2007

Over the last five years, high-content screening (HCS) has been increasingly adopted in early drug discovery, in order to investigate in detail cellular processes and quantify the effects of compounds on cellular pathways.

Since even a few screening plates may yield large volumes of images and data, the effective management of HCS information and its transformation into knowledge are major challenges. To this end, image storage solutions, software for interpretation of images and for the analysis of extracted data need to be combined, supporting an iterative (re-)analysis cycle towards consistent and biologically meaningful results and decisions.

In our case study a Transfluor GPCR assay was screened on the Cellomics® ArrayScan® VTI, image data was extracted using Cellomics BioApplications. The resulting HCS parameters were analyzed with the Genedata Screener software on different levels, from cell populations to complete plate sets.

We demonstrate how seamless integration of data management and analysis solutions - the possibility to move back and forth in the HCS data analysis process with minimum effort - facilitates the selection of relevant assay readouts and reliable quantification of cellular parameters, speeding up and improving hit selection and compound profiling by HCS.

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