Poster: High-Content Data Analysis for a Primary, Confirmation and Profiling Screen
SBS 2005

A major aim in optimizing the preclinical drug discovery process is to reduce the attrition rate on INDs entering the clinical phase. The simultaneous measurement of several biological responses incited by a compound is a big step forward in this respect. High-content screening (HCS) can in principle provide such information, but requires appropriate data analysis to deliver full value. This case study shows an example how HCS information is exploited by sophisticated data analysis.

A cell-based Endothelin-A-Receptor internalization assay was screened on the EVOscreen® uHTS system, combined with the high-content confocal imaging system Opera™. Image parameters were extracted from the images by the analysis tool Acapella™ and analyzed with the Genedata Screener® software. This combination of high-precision measurement, sophisticated image analysis, automated QC, and statistical analysis allows systematic discrimination of process artifacts from reliable signals and permits an early mechanistic classification of compounds.

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