Automated, high-speed microscopy and image analysis software have established High Content Screening (HCS) as a new experimental technology. It measures the reaction of cells to compounds or siRNA, quantifying phenotypes as well as intracellular concentrations and locations of multiple markers. This leads to new automatable assay systems of higher physiological relevance.
Genedata Screener exploits the full information behind the screen, while ensuring high data quality. Preparing them for biological interpretation, data from HCS readers are automatically integrated, assessed, standardized and structured. All Screener modules are "HCS-capable", processing HCS data at the same degree of automation, interactivity and ease-of-use as HTS data, and allowing full scalability towards high-throughput, high-content screens.
Benefits of Screener`s Application to HCS
- Processing rules geared towards complex HCS screens, with many parallel readouts
- Rapid QC based on raw signals or derived data, drill-down to original images
- Dose-response curve fitting of HCS screens, yielding multivariate IC50s per compound or siRNA
- Parallel biostatistical analysis of compound activity or siRNA knockdowns based on multiple readouts
- Filtering of compound or siRNA hits by multiple criteria
- High-performance data management
- Scalability to hundreds of thousands of wells measured in true HCS mode (several dozen readouts each)
Genedata Screener Solutions: High Content Analysis
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